Are the plates pre-coated? How do I store the remaining wells or plates if I can't use them all at once?
Yes. We provide pre-coated plates. The strips are detachable and it’s recommended to separate those wells needed for assay and store the rest at -20℃ without germ contamination.
What is the shelf life of OUR ELISA products?
6 months since the production date. Please refer to the box label for the shelf life.
How to store the ELISA Kit?
If the unopened kit is not supposed to be used up within 1 month, please store the Pre-coated Assay Plate, Standard, Biotin-antibody (100×), and Streptavidin-HRP (100×) at -20℃, other reagents at 2-8°C. , avoiding repeated freezing and thawing.
Opened reagents should be used as soon as possible. Unused parts should be stored properly and the date of opening should be recorded.
How long can I store lyophilized standards after dilution?
It needs to be used within 24 hours.
Q: Can I use antibodies or reagents from different manufacturers?
It is usually not recommended to use reagents from different manufacturers as they may be incompatible with other reagents in the Kit and may lead to inaccurate results.
How can I ensure reliable results from ELISA experiments?
1) Ensure the linear range of the standard curve; 2) follow instructions closely and maintain reagent consistency; 3) perform duplicate experiments to increase the reliability of results.
How are ELISA results expressed?
Typically, ELISA results can be expressed as optical density (OD) values, which are calculated and converted to the concentration of the target molecule (e.g. ng/mL or pg/mL).
What should I do if the results are abnormal?
1)Check the reagent and sample preparation steps to ensure that there is no mishandling; 2) ensure that the reagents have not expired and check that the storage conditions are as required; 3) consider repeating the experiment if the results deviate significantly.
What is a common misconception in ELISA experiments?
1)Not mixing samples or reagents sufficiently; 2) ignoring the effect of temperature and time on the experimental results; 3) inappropriate choice of standard curve range.
What is the reason for the low or high signal intensity?
Low signal: This may be related to low sample concentration, insufficient reaction time, or reagent inactivation.
High signal: This may be due to high sample concentration, improper operation, etc.
How to deal with ELISA waste?
Waste generated from ELISA experiments (e.g., samples, reagent tubes, etc.) should be disposed of under biosafety regulations to ensure that they do not pose a hazard to the environment or health.
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