| LPO

Lactoperoxidase (LPO) is a peroxidase enzyme secreted from mammary, salivary, tears and other mucosal glands including the lungs, bronchii and nose that function as a natural, first line of defense against bacteria and viral agents.Lactoperoxidase is a member of the heme peroxidase family of enzymes.Lactoperoxidase catalyzes the oxidation of several inorganic and many organic substrates by hydrogen peroxide.Lactoperoxidase rapidly oxidizes iodide and slowly oxidizes bromide and is designated a haloperoxidase. Another important substrate is the pseudo-halide thiocyanate. The oxidized products display potent, non-specific bactericidal and antiviral activities, including destruction of the influenza virus.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (ng/mL)	10	5	2.5	1.25	0.625	0.312	0.156	0
OD	2.11	1.26	0.8	0.5	0.33	0.24	0.19	0.08
Corrected OD	2.03	1.18	0.72	0.42	0.25	0.16	0.11	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Sheep LPO spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	84-101	95
Plasma(n=8)	92-105	102
Breast Milk(n=8)	88-106	96

 

| Linearity

Samples were spiked with high concentrations of Sheep LPO and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Serum(n=4)	Plasma(n=4)	Breast Milk(n=4)
1 : 2	Range (%)	84-95	88-96	92-106
	Average (%)	91	93	98
1 : 4	Range (%)	89-103	87-108	90-104
	Average (%)	94	97	95