| NT-ProANP

N-terminal pro-brain natriuretic peptide (NT-proBNP or BNPT) is a precursor to an N-terminal inactive protein with 76 amino acids, which is cleaved from the molecule as brain natriuretic peptide. Blood levels of both BNP and NT-proBNP can be used to screen for, and diagnose, acute congestive heart failure (CHF) and can be used to determine the prognosis of heart failure, as both markers are usually higher in patients with a poorer prognosis. Plasma concentrations are also usually increased in patients with asymptomatic or symptomatic left ventricular dysfunction and are associated with coronary artery disease and myocardial ischemia.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (ng/mL)	20	10	5	2.5	1.25	0.62	0.31	0
OD	2.59	1.57	0.94	0.64	0.38	0.26	0.23	0.08
Corrected OD	2.51	1.49	0.86	0.56	0.3	0.18	0.15	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Rat NT-ProANP spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	84-101	96
Plasma(n=8)	92-105	101
Cell Culture Supernates(n=8)	96-108	105

 

| Linearity

Samples were spiked with high concentrations of Rat NT-ProANP and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Serum(n=4)	Plasma(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	84-95	88-96	90-110
	Average (%)	91	93	96
1 : 4	Range (%)	89-103	87-108	105-115
	Average (%)	94	98	108