Rat Growth Associated Protein 43 (GAP43) ELISA Kit

Cat.#:

JL13777

Regulatory Status:

RUO

Alternative:

F1; B-50; PP46; Neuron Growth-Associated Protein 43; Neuromodulin; Axonal Membrane Protein GAP-43; Protein F1; Calmodulin-Binding Protein P-57; Neural Phosphoprotein B-50;GAP43

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

78.12-5000pg/mL

Sensitivity:

39.2pg/mL

Specificity:

This kit recognizes Rat GAP43 in samples. No significant cross-reactivity or interference between Rat GAP43 and analogues was observed.

Sample Type:

tissue homogenates;cell lysates and other biological fluids

Assay Procedure:

Research Area:

Neuroscience

Size & Price:

Human A1BG Polyclonal Antibody

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Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| GAP43

Growth Associated Protein 43 (GAP43) is a protein encoded by the GAP43 gene. GAP43 is called a "growth" or "plasticity" protein because it is expressed at high levels in neuronal growth cones during development[6] and axonal regeneration, and it is phosphorylated after long-term potentiation and after learning. GAP43 is a crucial component of the axon and presynaptic terminal. Its null mutation leads to death within days after birth, due to axon pathfinding defects.

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

Concentration (pg/mL)	5000	2500	1250	625	312.5	156.25	78.12	0
OD	2.07	1.48	0.94	0.65	0.43	0.31	0.24	0.08
Corrected OD	1.99	1.4	0.86	0.57	0.35	0.23	0.16	-

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Rat GAP43 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Tissue Homogenates(n=8)	84-103	98
Cell Lysates(n=8)	93-105	102

| Linearity

Samples were spiked with high concentrations of Rat GAP43 and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Tissue Homogenates(n=4)	Cell Lysates(n=4)
1 : 2	Range (%)	84-95	86-96
1 : 2	Average (%)	91	92
1 : 4	Range (%)	89-103	87-108
1 : 4	Average (%)	100	102