| NFE2L2

Nuclear factor erythroid 2-related factor 2 (NRF2), also known as nuclear factor erythroid-derived 2-like 2, is a transcription factor that is encoded by the NFE2L2 gene.NRF2 is a basic leucine zipper (bZIP) protein that may regulate the expression of antioxidant proteins that protect against oxidative damage triggered by injury and inflammation, according to preliminary research.In vitro, NRF2 binds to antioxidant response elements (AREs) in the promoter regions of genes encoding cytoprotective proteins.NRF2 induces the expression of heme oxygenase 1 in vitro leading to an increase in phase II enzymes.NRF2 also inhibits the NLRP3 inflammasome.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (ng/mL)	10	5	2.5	1.25	0.62	0.31	0.15	0
OD	2.18	1.57	1.03	0.62	0.42	0.32	0.26	0.1
Corrected OD	2.08	1.47	0.93	0.52	0.32	0.22	0.16	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Rat NFE2L2 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Tissue Homogenates(n=8)	84-102	97
Cell Lysates(n=8)	92-106	102

 

| Linearity

Samples were spiked with high concentrations of Rat NFE2L2 and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Tissue Homogenates(n=4)	Cell Lysates(n=4)
1 : 2	Range (%)	84-97	88-96
	Average (%)	92	93
1 : 4	Range (%)	89-103	87-108
	Average (%)	94	97