Home > Sandwich ELISA Kits > Rat Gamma-amino-N-butyrate transaminase (GABAT) ELISA Kit

Product Name:

Rat Gamma-amino-N-butyrate transaminase (GABAT) ELISA Kit

Cat.#:

JL47786

Regulatory Status:

RUO

Alternative:

Abat; beta-AlaAT I;4-aminobutyrate transaminase;GABA transaminase;4-aminobutyrate aminotransferase;

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.156-10ng/mL

Sensitivity:

0.079ng/mL

Specificity:

This kit recognizes Rat GABAT in samples. No significant cross-reactivity or interference between Rat GABAT and analogues was observed.

Sample Type:

Serum;plasma;tissue homogenates;cell lysates and other biological fluids

Assay Procedure:

Research Area:

Cell Biology

Size & Price:

Human A1BG Polyclonal Antibody

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Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| GABAT

Gamma-amino-N-butyrate transaminase (GABAT) is a protein that in humans is encoded by the ABAT gene.This gene is located in chromosome 16 at position of 13.2. This gene goes by a number of names, including, GABA transaminase, GABAT, 4-aminobutyrate transaminase, NPD009 etc.It is mainly and abundant located in neuronal tissues.4-Aminobutyrate aminotransferase belongs to group of pyridoxal 5-phosphate-dependent enzyme which activates a large portion giving reaction to amino acids.ABAT is made up of two monomers of enzymes where each subunit has a molecular weight of 50kDa.It is identified that almost tierce of human synapses have GABA.GABA is a neurotransmitter that has different roles in different regions of the central and peripheral nervous systems. It can be found also in some tissues that do not have neurons.

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

Concentration (ng/mL)	10	5	2.5	1.25	0.625	0.312	0.156	0
OD	2.26	1.45	0.85	0.55	0.36	0.27	0.19	0.07
Corrected OD	2.19	1.38	0.78	0.48	0.29	0.2	0.12	-

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Rat GABAT spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	84-101	96
Plasma(n=8)	92-105	103
Cell Lysates(n=8)	96-108	105

| Linearity

Samples were spiked with high concentrations of Rat GABAT and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Serum(n=4)	Plasma(n=4)	Cell Lysates(n=4)
1 : 2	Range (%)	84-95	88-96	90-110
1 : 2	Average (%)	90	93	96
1 : 4	Range (%)	89-103	87-108	105-113
1 : 4	Average (%)	94	98	107