| CKLF1

Chemokine-like factor (CKLF) is a member of the CKLF-like MARVEL transmembrane domain-containing family of proteins that is encoded by the CKLF gene. CKLF1 is the first member of the CKLF-like MARVEL transmembrane domain-containing family of proteins to be defined and the most investigated of its four isoforms. Studies conducted in freshly isolated cells, cultured cells, animals, and tissue samples indicate that CKLF1 is a chemokine-like chemotactic factor that acts through the CCR4 receptors on human CD4+ Th2 lymphocytes, neutrophils, monocytes, macrophages, dendritic cells, and perhaps other CCR4-receptor bearing cells.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (pg/mL)	500	250	125	62.5	31.2	15.6	7.8	0
OD	2.22	1.56	0.92	0.63	0.42	0.25	0.18	0.08
Corrected OD	2.14	1.48	0.84	0.55	0.34	0.17	0.1	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Rat CKLF1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	84-101	96
Plasma(n=8)	92-105	102

 

| Linearity

Samples were spiked with high concentrations of Rat CKLF1 and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Serum(n=4)	Plasma(n=4)
1 : 2	Range (%)	84-95	88-96
	Average (%)	93	90
1 : 4	Range (%)	89-104	87-108
	Average (%)	99	102