Rat Sirtuin 3 (SIRT3) ELISA Kit

Cat.#:

JL50176

Regulatory Status:

RUO

Alternative:

SIR2L3; Silent Mating Type Information Regulation 2 Homolog 3; NAD-dependent protein deacetylase sirtuin-3, mitochondrial; SIR2-like protein 3; Regulatory protein SIR2 homolog 3

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.156-10ng/mL

Sensitivity:

0.079ng/mL

Specificity:

This kit recognizes Rat SIRT3 in samples. No significant cross-reactivity or interference between Rat SIRT3 and analogues was observed.

Sample Type:

Tissue homogenates, cell lysates and other biological fluids

Assay Procedure:

Research Area:

Signal Transduction

Size & Price:

Human A1BG Polyclonal Antibody

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Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| SIRT3

NAD-dependent deacetylase sirtuin-3, mitochondrial also known as SIRT3 is a protein that in humans is encoded by the SIRT3 gene. SIRT3 is member of the mammalian sirtuin family of proteins, which are homologs to the yeast Sir2 protein. SIRT3 exhibits NAD+-dependent deacetylase activity.Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes, and the protein encoded by this gene is included in class I of the sirtuin family.

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

Concentration (ng/mL)	10	5	2.5	1.25	0.625	0.312	0.156	0
OD	2.24	1.55	0.98	0.52	0.32	0.21	0.17	0.08
Corrected OD	2.16	1.47	0.9	0.44	0.24	0.13	0.09	-

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Rat SIRT3 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Tissue Homogenates(n=8)	84-101	96
Cell Culture Supernates(n=8)	96-108	105

| Linearity

Samples were spiked with high concentrations of Rat SIRT3 and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Tissue Homogenates(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	88-96	90-110
1 : 2	Average (%)	93	96
1 : 4	Range (%)	87-107	105-115
1 : 4	Average (%)	97	108