| INOS

Nitric Oxide Synthase 2, Inducible，also known as NOS2，is an enzyme encoded by the NOS2 gene. Resting immune cells lack the inducible NO synthase (iNOS or NOS2), the enzyme that synthesizes NO. However, a variety of extracellular stimuli can activate distinct signaling pathways that converge to initiate expression of NOS2. Nitric oxide is a reactive free radical mediating in neurotransmission, antimicrobial and antitumoral activities.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (ng/mL)	100	50	25	12.5	6.25	3.12	1.56	0
OD	2.57	1.98	1.41	1.22	0.78	0.45	0.27	0.09
Corrected OD	2.48	1.89	1.32	1.13	0.69	0.36	0.18	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Rat INOS spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Tissue Homogenates(n=8)	82-101	96
Cell Lysates(n=8)	92-105	102
Cell Culture Supernates(n=8)	96-109	105

 

| Linearity

Samples were spiked with high concentrations of Rat INOS and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Tissue Homogenates(n=4)	Cell Lysates(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	84-95	88-97	90-111
	Average (%)	90	93	96
1 : 4	Range (%)	89-103	87-108	105-115
	Average (%)	92	98	108