| FⅡ

Prothrombin (coagulation factor II) is encoded by the F2-gene. The molecular weight of prothrombin is approximately 72, Da. The catalytic domain is released from prothrombin fragment 1.2 to create the active enzyme thrombin, which has a molecular weight of 36, Da. Structurally, it is a member of the large PA clan of proteases. It is proteolytically cleaved during the clotting process by the prothrombinase enzyme complex to form thrombin.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (ng/mL)	250	125	62.5	31.2	15.6	7.8	3.9	0
OD	2.27	1.55	0.96	0.55	0.37	0.26	0.19	0.09
Corrected OD	2.18	1.46	0.87	0.46	0.28	0.17	0.1	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Rat FⅡ spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	92-105	102

 

| Linearity

Samples were spiked with high concentrations of Rat FⅡ and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Serum(n=4)
1 : 2	Range (%)	84-95
	Average (%)	90
1 : 4	Range (%)	89-103
	Average (%)	94