Pig Granulysin (GNLY) ELISA Kit

Cat.#:

JL54068

Regulatory Status:

RUO

Alternative:

NKG5; LAG-2; TLA519; T-Lymphocyte Activation Gene 519

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.23-15ng/mL

Sensitivity:

0.09ng/mL

Specificity:

This kit recognizes Pig GNLY in samples. No significant cross-reactivity or interference between Pig GNLY and analogues was observed.

Sample Type:

Serum;plasma;tissue homogenates;cell lysates;cell culture supernates and other biological fluids

Assay Procedure:

Research Area:

Microbiology

Size & Price:

Human A1BG Polyclonal Antibody

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Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| GNLY

Granulysin (GNLY) is a protein expressed in most mammals which functions as an antimicrobial peptide released by killer lymphocytes in cytotoxic granules.It is a pore-forming peptide, as it can puncture a microbial cell wall, allowing for other death-inducing enzymes to enter the microbe and cause microptosis.GNLY is inhibited by cholesterol, and is most effective in helping to kill cholesterol-deficient microbes.

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Pig GNLY spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Tissue Homogenates(n=8)	85-101	96
Cell Lysates(n=8)	92-105	102

| Linearity

Samples were spiked with high concentrations of Pig GNLY and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Tissue Homogenates(n=4)	Cell Lysates(n=4)
1 : 2	Range (%)	84-96	88-96
1 : 2	Average (%)	91	93
1 : 4	Range (%)	84-105	86-108
1 : 4	Average (%)	95	98