Mouse Neuregulin 4 (NRG4) ELISA Kit

Cat.#:

JL15732

Regulatory Status:

RUO

Alternative:

HRG4; Pro-NRG4; Pro-neuregulin-4, membrane-bound isoform

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.31-20ng/mL

Sensitivity:

0.15ng/mL

Specificity:

This kit recognizes Mouse NRG4 in samples. No significant cross-reactivity or interference between Mouse NRG4 and analogues was observed.

Sample Type:

Serum, plasma, tissue homogenates and other biological fluids

Assay Procedure:

Research Area:

Neuroscience

Size & Price:

Human A1BG Polyclonal Antibody

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Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| NRG4

Neuregulin 4 also known as NRG4 is a member of the neuregulin protein family which is encoded by the NRG4 gene.The neuregulins, including NRG4, activate erb-b2 receptor tyrosine kinase 4 (ERBB4) to initiating cell signaling through cytosolic tyrosine phosphorylation.

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

Concentration (ng/mL)	20	10	5	2.5	1.25	0.62	0.31	0
OD	2.49	1.95	1.22	0.7	0.45	0.29	0.21	0.08
Corrected OD	2.41	1.87	1.14	0.62	0.37	0.21	0.13	-

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Mouse NRG4 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	84-101	96
Plasma(n=8)	92-105	102
Cell Culture Supernates(n=8)	96-108	105

| Linearity

Samples were spiked with high concentrations of Mouse NRG4 and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Serum(n=4)	Plasma(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	84-95	88-96	90-110
1 : 2	Average (%)	91	92	96
1 : 4	Range (%)	89-103	87-108	105-115
1 : 4	Average (%)	94	98	108