| CASP3

Caspase-3 is a caspase protein that interacts with caspase-8 and caspase-9. It is encoded by the CASP3 gene. CASP3 orthologs[4] have been identified in numerous mammals for which complete genome data are available. Unique orthologs are also present in birds, lizards, lissamphibians, and teleosts.The CASP3 protein is a member of the cysteine-aspartic acid protease (caspase) family.Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (ng/mL)	10	5	2.5	1.25	0.62	0.31	0.15	0
OD	2.44	1.87	1.29	0.83	0.58	0.38	0.27	0.08
Corrected OD	2.36	1.79	1.21	0.75	0.5	0.3	0.19	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Mouse CASP3 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Cell Culture Supernates(n=8)	96-108	105

 

| Linearity

Samples were spiked with high concentrations of Mouse CASP3 and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Cell Culture Supernates(n=4)
1 : 2	Range (%)	90-110
	Average (%)	95
1 : 4	Range (%)	105-115
	Average (%)	108