Home > Sandwich ELISA Kits > Mouse TEK Tyrosine Kinase, Endothelial (Tie2) ELISA Kit

Product Name:

Mouse TEK Tyrosine Kinase, Endothelial (Tie2) ELISA Kit

Cat.#:

JL10663

Regulatory Status:

RUO

Alternative:

CD202b; CD202-B; TEK; VMCM1; Tyrosine Kinase With Immunoglobulin Like And EGF Like Domains 2; Venous Malformations Multiple Cutaneous And Mucosal

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.312-20ng/mL

Sensitivity:

0.151ng/mL

Specificity:

This kit recognizes Mouse Tie2 in samples. No significant cross-reactivity or interference between Mouse Tie2 and analogues was observed.

Sample Type:

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Procedure:

Research Area:

Immunology

Size & Price:

Human A1BG Polyclonal Antibody

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Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| Tie2

Angiopoietin-1 receptor also known as CD22B (cluster of differentiation 22B) is a protein encoded by the TEK gene. Also known as TIE2, it is an angiopoietin receptor. This receptor possesses a unique extracellular domain containing 2 immunoglobulin-like loops separated by 3 epidermal growth factor-like repeats that are connected to 3 fibronectin type III-like repeats. Defects in TEK are associated with inherited venous malformations. The TEK signaling pathway appears to be critical for endothelial cell-smooth muscle cell communication in venous morphogenesis.

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

Concentration (ng/mL)	20	10	5	2.5	1.25	0.625	0.312	0
OD	2.28	1.22	0.74	0.52	0.31	0.24	0.19	0.08
Corrected OD	2.2	1.14	0.66	0.44	0.23	0.16	0.11	-

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Mouse Tie2 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	84-102	95
Plasma(n=8)	92-106	103
Cell Culture Supernates(n=8)	96-108	105

| Linearity

Samples were spiked with high concentrations of Mouse Tie2 and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Serum(n=4)	Plasma(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	84-95	88-95	90-110
1 : 2	Average (%)	91	93	96
1 : 4	Range (%)	89-103	87-108	105-114
1 : 4	Average (%)	94	98	108