| GZMK

Granzyme K (GrK) is a protein that is encoded by the GZMK gene on chromosome 5. Granzymes are a family of serine proteases which have various intracellular and extracellular roles. GrK is found in granules of natural killer (NK) cells and cytotoxic T lymphocytes (CTLs), and is traditionally described as being cytotoxic towards targeted foreign, infected, or cancerous cells. NK cells and CTLs can induce apoptosis through the granule secretory pathway, which involves the secretion of granzymes along with perforin at immunological synapses.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (ng/mL)	10	5	2.5	1.25	0.62	0.31	0.15	0
OD	2.44	1.87	1.29	0.83	0.58	0.38	0.27	0.08
Corrected OD	2.36	1.79	1.21	0.75	0.5	0.3	0.19	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Mouse GZMK spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	84-101	96
Plasma(n=8)	92-105	101
Cell Lysates(n=8)	96-108	105

 

| Linearity

Samples were spiked with high concentrations of Mouse GZMK and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Serum(n=4)	Plasma(n=4)	Cell Lysates(n=4)
1 : 2	Range (%)	84-95	88-96	90-110
	Average (%)	91	92	96
1 : 4	Range (%)	89-103	87-108	105-115
	Average (%)	94	98	108