| PECAM1

Platelet endothelial cell adhesion molecule (PECAM-1) also known as cluster of differentiation 31 (CD31) is a protein that is encoded by the PECAM1 gene found on chromosome17q23.3.PECAM-1 plays a key role in removing aged neutrophils from the body.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (ng/mL)	20	10	5	2.5	1.25	0.62	0.31	0
OD	2.38	1.8	1.28	0.89	0.6	0.45	0.33	0.09
Corrected OD	2.29	1.71	1.19	0.8	0.51	0.36	0.24	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Mouse PECAM1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Plasma(n=8)	95-105	104

 

| Linearity

Samples were spiked with high concentrations of Mouse PECAM1 and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Plasma(n=4)
1 : 2	Range (%)	87-96
	Average (%)	94
1 : 4	Range (%)	87-108
	Average (%)	97