Product Name:
Mouse Low Density LipoProtein Cholesterol (LDL-C) ELISA Kit
Cat.#:
JL20313
Regulatory Status:
Alternative:
low density lipoprotein cholesterol
Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
3.12-200ng/mL
Sensitivity:
1.56ng/mL
Specificity:
This kit recognizes Mouse LDL-C in samples. No significant cross-reactivity or interference between Mouse LDL-C and analogues was observed.
Sample Type:
Serum;plasma;tissue homogenates;cell lysates;cell culture supernates and other biological fluids
Assay Procedure:
Research Area:
Cardiovascular Biology
Product Datasheets
| LDL-C
Very low-density lipoprotein cholesterol (VLDL-C) is primarily synthesized in the liver and plays a crucial role in transporting endogenously produced triglycerides (TGs). Its degradation is influenced by various factors, including diet, enterhepatic circulation, capillary endothelial function, and hormones such as adrenaline. VLDL (very low-density lipoprotein) is a lipoprotein particle produced in the liver, carrying triglycerides and cholesterol. VLDL-C specifically refers to the cholesterol content within VLDL particles, serving as an important biomarker for lipid metabolism assessment.
| Repeatability
Intra-assay Precision (Precision within an assay):CV% < 10%; Inter-assay Precision (Precision between assays):CV% < 10%| Recovery
The recovery of Mouse LDL-C spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.| Sample Type | Range(%) | Average Recovery(%) |
| Serum(n=8) | 84-101 | 96 |
| Plasma(n=8) | 92-105 | 102 |
| Cell Culture Supernates(n=8) | 96-107 | 104 |
| Linearity
Samples were spiked with high concentrations of Mouse LDL-C and diluted with Universal Diluent to produce samples with values within the range of the assay.| Serum(n=4) | Plasma(n=4) | Cell Culture Supernates(n=4) | ||
| 1 : 2 | Range (%) | 84-95 | 87-96 | 90-110 |
| Average (%) | 91 | 92 | 96 | |
| 1 : 4 | Range (%) | 89-103 | 87-108 | 105-115 |
| Average (%) | 94 | 98 | 108 |
Size
Quantity
Price ($)
48T
1
360 / 360
96T
1
460 / 460


