Product Name:
Mouse Apoptosis Signal Regulating Kinase 1 (ASK1) ELISA Kit
Cat.#:
JL52220
Regulatory Status:
Alternative:
MAP3-K5; MAP3K5; ASK1; MAPKKK5; MEKK5; Mitogen-Activated Protein Kinase Kinase Kinase 5; MAPK/ERK kinase kinase 5
Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
78.12-5000pg/mL
Sensitivity:
38pg/mL
Specificity:
This kit recognizes Mouse ASK1 in samples. No significant cross-reactivity or interference between Mouse ASK1 and analogues was observed.
Sample Type:
tissue homogenates;cell lysates and other biological fluids
Assay Procedure:
Research Area:
Metabolism
Size & Price: 
Product Datasheets
Instruction: 
| ASK1
Apoptosis Signal Regulating Kinase 1 (ASK1) also known as mitogen-activated protein kinase 5 (MAP3K5) is a member of MAP kinase family and as such a part of mitogen-activated protein kinase pathway. It activates c-Jun N-terminal kinase (JNK) and p38 mitogen-activated protein kinases in a Raf-independent fashion in response to an array of stresses such as oxidative stress, endoplasmic reticulum stress and calcium influx. ASK1 has been found to be involved in cancer, diabetes, rheumatoid arthritis, cardiovascular and neurodegenerative diseases.
| Repeatability
Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%| Recovery
The recovery of Mouse ASK1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.| Sample Type | Range(%) | Average Recovery(%) |
| Tissue Homogenates(n=8) | 84-101 | 96 |
| Cell Lysates(n=8) | 92-105 | 102 |
| Linearity
Samples were spiked with high concentrations of Mouse ASK1 and diluted with Universal Diluent to produce samples with values within the range of the assay.| Tissue Homogenates(n=4) | Cell Lysates(n=4) | ||
| 1 : 2 | Range (%) | 84-95 | 88-96 |
| Average (%) | 91 | 93 | |
| 1 : 4 | Range (%) | 89-103 | 87-108 |
| Average (%) | 94 | 98 |
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