Mouse Purinergic Receptor P2Y12 (P2RY12) ELISA Kit

Cat.#:

JL19501

Regulatory Status:

RUO

Alternative:

ADPG-R; HORK3; P2T(AC); P2Y(AC); P2Y(ADP); P2Y(cyc); P2Y12; SP1999; ADP-glucose receptor; P2Y12 platelet ADP receptor

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.156-10ng/mL

Sensitivity:

0.071ng/mL

Specificity:

This kit recognizes Mouse P2RY12 in samples. No significant cross-reactivity or interference between Mouse P2RY12 and analogues was observed.

Sample Type:

Serum;plasma;tissue homogenates;cell lysates;cell culture supernates and other biological fluids

Assay Procedure:

Research Area:

Neuroscience

Size & Price:

Human A1BG Polyclonal Antibody

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Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| P2RY12

P2Y12 is a chemoreceptor for adenosine diphosphate (ADP) that belongs to the Gi class of a group of G protein-coupled (GPCR) purinergic receptors. This P2Y receptor family has several receptor subtypes with different pharmacological selectivity, which overlaps in some cases, for various adenosine and uridine nucleotides. The P2Y12 receptor is involved in platelet aggregation and is thus a biological target for the treatment of thromboembolisms and other clotting disorders. Two transcript variants encoding the same isoform have been identified for this gene.

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Mouse P2RY12 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	84-101	96
Plasma(n=8)	92-105	102
Cell Culture Supernates(n=8)	96-108	105

| Linearity

Samples were spiked with high concentrations of Mouse P2RY12 and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Serum(n=4)	Plasma(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	84-95	88-96	90-111
1 : 2	Average (%)	91	93	96
1 : 4	Range (%)	89-102	87-108	105-115
1 : 4	Average (%)	94	98	108