| C3c

The complement fragment C3c consists of three polypeptide chains: the C3c β-chain (residues 23-667), C3c α-chain fragment 1 (residues 749-954), and C3c α-chain fragment 2 (residues 1321-1663), which are interconnected through disulfide bonds. As a central component of both complement activation pathways, C3c undergoes specific proteolytic cleavage to form C3 convertase. The cleavage of C3 releases C3a and C3b fragments, with the latter serving as a key component of the alternative C3 convertase. Circulating C3 levels may become significantly reduced either due to decreased synthesis or increased consumption during complement activation.

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Mouse C3c spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	84-102	97
Plasma(n=8)	92-105	102
Cell Culture Supernates(n=8)	96-108	105

 

| Linearity

Samples were spiked with high concentrations of Mouse C3c and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Serum(n=4)	Plasma(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	84-95	88-96	90-111
	Average (%)	91	93	97
1 : 4	Range (%)	89-103	87-108	105-115
	Average (%)	94	98	108