| GAD67

Glutamate decarboxylase 67 is an enzyme that catalyzes the decarboxylation of glutamate to gamma-aminobutyric acid (GABA) and carbon dioxide (CO2). Involved in several processes, including cellular response to serotonin; gamma-aminobutyric acid biosynthetic process; and positive regulation of locomotion involved in locomotory behavior. Located in neuronal cell body and presynaptic active zone. Used to study diabetes mellitus.It is identified as biomarker of several diseases, including autism spectrum disorder; drug psychosis; morphine dependence; transient cerebral ischemia; and withdrawal disorder. Human ortholog(s) of this gene implicated in several diseases, including alcohol dependence; amphetamine abuse; developmental and epileptic encephalopathy 89; drug psychosis; and heroin dependence.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (ng/mL)	50	25	12.5	6.25	3.12	1.56	0.78	0
OD	2.29	1.29	0.71	0.43	0.29	0.2	0.18	0.07
Corrected OD	2.22	1.22	0.64	0.36	0.22	0.13	0.11	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Mouse GAD67 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Tissue Homogenates(n=8)	92-104	101
Cell Culture Supernates(n=8)	96-108	105

 

| Linearity

Samples were spiked with high concentrations of Mouse GAD67 and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Tissue Homogenates(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	84-96	90-110
	Average (%)	92	96
1 : 4	Range (%)	89-103	105-115
	Average (%)	94	108