| CXCL15

Chemokine (C-X-C motif) ligand 15 (CXCL15) is a small cytokine belonging to the CXC chemokine family. This chemokine is also known under the name lungkine. CXCL15 is an ELR+ CXC chemokine (it contains the amino acid sequence E-L-R immediately before its CXC motif) that recruits neutrophils during inflammation of the lungs. It is highly abundant in epithelial cells of the lung,[2] and can also be found in other mucosal organs such as the urogenital and gastrointestinal tracts, and in endocrine organs like the adrenal gland. The gene for CXCL15 is found on mouse chromosome 5.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (pg/mL)	8000	4000	2000	1000	500	250	125	0
OD	2.6	1.51	0.78	0.46	0.32	0.24	0.2	0.09
Corrected OD	2.51	1.42	0.69	0.37	0.23	0.15	0.11	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Mouse CXCL15 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	84-101	96
Plasma(n=8)	92-105	102

 

| Linearity

Samples were spiked with high concentrations of Mouse CXCL15 and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Serum(n=4)	Plasma(n=4)
1 : 2	Range (%)	84-95	87-96
	Average (%)	91	93
1 : 4	Range (%)	89-103	87-108
	Average (%)	94	98