Mouse Interleukin 33 (IL-33) ELISA Kit

Cat.#:

JL20264

Regulatory Status:

RUO

Alternative:

DV27; C9ORF26; IL1F11; NFHEV; Interleukin-1 Family, Member 11; Nuclear factor from high endothelial venules

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

6.25-400pg/mL

Sensitivity:

1.84pg/mL

Specificity:

This kit recognizes Mouse IL-33 in samples. No significant cross-reactivity or interference between Mouse IL-33 and analogues was observed.

Sample Type:

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Procedure:

Research Area:

Immunology

Size & Price:

Human A1BG Polyclonal Antibody

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Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| IL-33

Interleukin 33 (IL-33) is a protein that is encoded by the IL33 gene.Interleukin 33 is a member of the IL-1 family that potently drives production of T helper-2 (Th2)-associated cytokines (e.g., IL-4). IL33 is a ligand for ST2 (IL1RL1), an IL-1 family receptor that is highly expressed on Th2 cells, mast cells and group 2 innate lymphocytes.

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

Concentration (pg/mL)	400	200	100	50	25	12.5	6.25	0
OD	2.36	1.36	0.8	0.48	0.34	0.28	0.23	0.09
Corrected OD	2.27	1.27	0.71	0.39	0.25	0.19	0.14	-

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Mouse IL-33 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	83-101	96
Plasma(n=8)	93-105	102
Cell Culture Supernates(n=8)	97-108	105

| Linearity

Samples were spiked with high concentrations of Mouse IL-33 and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Serum(n=4)	Plasma(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	84-95	88-96	92-110
1 : 2	Average (%)	91	93	96
1 : 4	Range (%)	90-103	87-108	105-115
1 : 4	Average (%)	94	98	109