| AP13

Apelin-13 is considered the most functional subtype of the Apelin family, and it actively participates in regulating bone marrow mesenchymal stem cell (BMSC) apoptosis, osteoblast differentiation, glucose and lipid metabolism, and other physiological processes, which means that it has an important role in the prevention and treatment of metabolic diseases such as obesity, diabetes and osteoporosis.Apelin-13 can also serve as a “motion-sensitive factor” and can be used to increase the potential of exercise. Long-term exercise can also promote the expression of Apelin-13 in the serum of obese individuals, suggesting that exercise might regulate metabolic diseases through Apelin-13.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (pg/mL)	2000	1000	500	250	125	62.5	31.2	0
OD	2.11	1.26	0.8	0.5	0.33	0.24	0.19	0.08
Corrected OD	2.03	1.18	0.72	0.42	0.25	0.16	0.11	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Mouse AP13 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	84-103	98
Plasma(n=8)	92-105	102

 

| Linearity

Samples were spiked with high concentrations of Mouse AP13 and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Serum(n=4)	Plasma(n=4)
1 : 2	Range (%)	84-95	88-98
	Average (%)	91	95
1 : 4	Range (%)	89-103	88-108
	Average (%)	94	98