| proBDNF

Brain-derived neurotrophic factor (BDNF) belongs to the family of neurotrophins and stimulates neuronal growth and differentiation, synaptogenesis, and neural plasticity.Brain-derived neurotrophic factor is synthesized as an isoform termed pro-BDNF, which is reported to have a molecular mass of 28-32 kDa, where the canonical sequence is 28 kDa.Pro-BDNF can be cleaved, both intra- and extracellularly, to the 14-kDa mature BDNF (mBDNF or conventionally just BDNF) and a 17-kDa pro-peptide.Pro-BDNF is biologically active and binds to the p75NTR receptor, while mBDNF exhibits substrate specificity for tyrosine receptor kinase B (TrkB).
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (pg/mL)	2000	1000	500	250	125	62.5	31.2	0
OD	2.22	1.56	0.92	0.63	0.42	0.25	0.18	0.08
Corrected OD	2.14	1.48	0.84	0.55	0.34	0.17	0.1	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Mouse proBDNF spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	84-103	98
Plasma(n=8)	92-105	102
Cell Culture Supernates(n=8)	96-108	105

 

| Linearity

Samples were spiked with high concentrations of Mouse proBDNF and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Serum(n=4)	Plasma(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	84-95	88-98	90-110
	Average (%)	91	95	96
1 : 4	Range (%)	89-103	87-108	105-115
	Average (%)	94	98	108