400-0066-400

Mouse Glucocorticoid Receptor Alpha (GR-α) ELISA Kit

Home  >  Sandwich ELISA Kits  >  Mouse Glucocorticoid Receptor Alpha (GR-α) ELISA Kit
Product Name:

Mouse Glucocorticoid Receptor Alpha (GR-α) ELISA Kit

Cat.#:

JL11259

Regulatory Status:
Alternative:

GR-A

Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
0.31-20ng/mL
Sensitivity:
0.1ng/mL
Specificity:
This kit recognizes Mouse GR-α in samples. No significant cross-reactivity or interference between Mouse GR-α and analogues was observed.
Sample Type:
Tissue homogenates, cell lysates and other biological fluids
Assay Procedure:
Assay Procedure
Research Area:
Signal Transduction
  • Mouse Glucocorticoid Receptor Alpha (GR-α) ELISA Kit
  • Mouse Glucocorticoid Receptor Alpha (GR-α) ELISA Kit
  • Mouse Glucocorticoid Receptor Alpha (GR-α) ELISA Kit
Size & Price: cart
×
Human A1BG Polyclonal Antibody
Email *
Message *
Please Sign In.
Product Datasheets
Instruction: Down Instruction


| GR-α

Glucocorticoid receptor alpha(GR-α) is one of the subtypes of glucocorticoid receptors. The glucocorticoid receptor(GR) is the receptor to which cortisol and other glucocorticoids bind. The GR is expressed in almost every cell in the body and regulates genes controlling the development, metabolism, and immune response. GR-α is the result of the same transcription of the glucocorticoid receptor gene splicing through different splicing methods. At the mRNA level, GR-α contains exons 1 to 8, including exons 9.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 
Concentration (ng/mL) 20 10 5 2.5 1.25 0.62 0.31 0
OD 2.64 1.78 1.09 0.64 0.41 0.31 0.23 0.09
Corrected OD 2.55 1.69 1 0.55 0.32 0.22 0.14 -
 





| Repeatability

Intra-assay Precision (Precision within an assay):CV% < 10%; Inter-assay Precision (Precision between assays):CV% < 10%
 

| Recovery

The recovery of Mouse GR-α spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 
Sample Type Range(%) Average Recovery(%)
Tissue Homogenates(n=8) 84-101 96
Cell Lysates(n=8) 92-105 101





 

| Linearity

Samples were spiked with high concentrations of Mouse GR-α and diluted with Universal Diluent to produce samples with values within the range of the assay.

 
    Tissue Homogenates(n=4) Cell Lysates(n=4)
1 : 2 Range (%) 84-95 88-96
Average (%) 91 93
1 : 4 Range (%) 89-103 87-108
Average (%) 94 98







 
Close
Sample Data
Close
Sample Data
Close
Sample Data
Size Quantity Price ($)
48T
1
360 / 360
96T
1
460 / 460
Top