Home > Sandwich ELISA Kits > Mouse Inhibitory Subunit Of NF Kappa B Alpha (IkBα) ELISA Kit

Product Name:

Mouse Inhibitory Subunit Of NF Kappa B Alpha (IkBα) ELISA Kit

Cat.#:

JL15701

Regulatory Status:

RUO

Alternative:

NFKBIA; IKB-A; MAD-3; NFKBI; Nuclear Factor Of Kappa Light Polypeptide Gene Enhancer In B-Cells Inhibitor,Alpha; Major histocompatibility complex enhancer-binding protein MAD3

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.31-20ng/mL

Sensitivity:

0.11ng/mL

Specificity:

This kit recognizes Mouse IkBα in samples. No significant cross-reactivity or interference between Mouse IkBα and analogues was observed.

Sample Type:

Tissue homogenates, cell lysates and other biological fluids.

Assay Procedure:

Research Area:

Signal Transduction

Size & Price:

Human A1BG Polyclonal Antibody

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Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| IkBα

Glucocorticoid Receptor Alpha (IκBα) is one member of a family of cellular proteins that function to inhibit the NF-κB transcription factor. IκBα inhibits NF-κB by masking the nuclear localization signals (NLS) of NF-κB proteins and keeping them sequestered in an inactive state in the cytoplasm. In addition, IκBα blocks the ability of NF-κB transcription factors to bind to DNA, which is required for NF-κB's proper functioning.

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

Concentration (ng/mL)	20	10	5	2.5	1.25	0.62	0.31	0
OD	2.07	1.48	0.94	0.65	0.43	0.31	0.24	0.08
Corrected OD	1.99	1.4	0.86	0.57	0.35	0.23	0.16	-

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Mouse IkBα spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Tissue Homogenates(n=8)	84-101	96
Cell Lysates(n=8)	92-105	102

| Linearity

Samples were spiked with high concentrations of Mouse IkBα and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Tissue Homogenates(n=4)	Cell Lysates(n=4)
1 : 2	Range (%)	84-95	88-97
1 : 2	Average (%)	91	93
1 : 4	Range (%)	89-103	87-108
1 : 4	Average (%)	94	98