| MUC5B

Mucin-5B (MUC-5B) is a protein that is encoded by the MUC5B gene. It is one of the five gel-forming mucins. It can be found in whole saliva, normal lung mucus, and cervical mucus. In some diseases, such as COPD, chronic rhinosinusitis (CRS), and H. pylori-associated gastric disease, the gene has been found to be upregulated, and this may be related to the pathogenesis of these conditions.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (ng/mL)	20	10	5	2.5	1.25	0.62	0.31	0
OD	2.32	1.89	1.4	0.97	0.58	0.37	0.22	0.07
Corrected OD	2.25	1.82	1.33	0.9	0.51	0.3	0.15	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Mouse MUC5B spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Tissue Homogenates(n=8)	84-101	96
Cell Lysates(n=8)	92-105	102
Cell Culture Supernates(n=8)	97-108	104

 

| Linearity

Samples were spiked with high concentrations of Mouse MUC5B and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Tissue Homogenates(n=4)	Cell Lysates(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	84-95	88-96	90-110
	Average (%)	91	93	96
1 : 4	Range (%)	89-103	87-108	105-115
	Average (%)	94	98	108