Home > Sandwich ELISA Kits > Mouse mechanistic target of rapamycin (mTOR) ELISA Kit

Product Name:

Mouse mechanistic target of rapamycin (mTOR) ELISA Kit

Cat.#:

JL21135

Regulatory Status:

RUO

Alternative:

FRAP; FRAP1; FRAP2; MTOR; RAFT1; RAPT1; FK506 Binding Protein 12 Rapamycin Associated Protein; Mammalian Target Of Rapamycin; FKBP12-Rapamycin Complex-Associated Protein 1; Rapamycin and FKBP12 target

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.16-10ng/mL

Sensitivity:

0.07ng/mL

Specificity:

This kit recognizes Mouse mTOR in samples. No significant cross-reactivity or interference between Mouse mTOR and analogues was observed.

Sample Type:

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Procedure:

Research Area:

Cancer

Size & Price:

Human A1BG Polyclonal Antibody

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Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| mTOR

The mammalian target of rapamycin (mTOR),also referred to as the mechanistic target of rapamycin, and sometimes called FK506-binding protein 12-rapamycin-associated protein 1 (FRAP1), is a kinase that is encoded by the MTOR gene.mTOR is a member of the phosphatidylinositol 3-kinase-related kinase family of protein kinases.mTOR links with other proteins and serves as a core component of two distinct protein complexes, mTOR complex 1 and mTOR complex 2, which regulate different cellular processes.

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

Concentration (ng/mL)	10	5	2.5	1.25	0.625	0.312	0.16	0
OD	2.56	1.45	0.8	0.48	0.33	0.23	0.2	0.08
Corrected OD	2.48	1.37	0.72	0.4	0.25	0.15	0.12	-

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Mouse mTOR spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Tissue Homogenates(n=8)	80-101	96
Cell Lysates(n=8)	92-105	102

| Linearity

Samples were spiked with high concentrations of Mouse mTOR and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Tissue Homogenates(n=4)	Cell Lysates(n=4)
1 : 2	Range (%)	84-96	88-97
1 : 2	Average (%)	91	93
1 : 4	Range (%)	89-103	87-108
1 : 4	Average (%)	95	97