| TPO

Thrombopoietin (TPO) is a glycoprotein hormone produced by the liver and kidney which regulates the production of platelets. TPO consists of two distinct domains, including the erythropoietin (EPO) -like domain composed of amino acid residues at positions 1 to 153 and the sugar-rich domain composed of amino acid residues at positions 154 to 332.TPO stimulates the production and differentiation of megakaryocytes, the bone marrow cells that bud off large numbers of platelets. The thrombopoietin gene is located on the long arm of chromosome 3 (q26.3-27). Abnormalities in this gene occur in some hereditary forms of thrombocytosis and in some cases of leukemia.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (pg/mL)	1000	500	250	125	62.5	31.25	15.62	0
OD	2.44	1.87	1.29	0.83	0.58	0.38	0.27	0.08
Corrected OD	2.36	1.79	1.21	0.75	0.5	0.3	0.19	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Mouse TPO spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	84-101	96
Plasma(n=8)	92-105	102
Cell Culture Supernates(n=8)	96-108	105

 

| Linearity

Samples were spiked with high concentrations of Mouse TPO and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Serum(n=4)	Plasma(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	84-95	88-96	90-110
	Average (%)	91	93	96
1 : 4	Range (%)	89-103	87-108	105-115
	Average (%)	94	98	108