| CKMB

Creatine kinase MB isoenzyme (CKMB) is a dimer composed of brain subunit (B) and muscle subunit (M). Normal tissues usually contain three isoenzymes, which are CK-BB (CK1), CK-MB (CK2) and CK-MM (CK3) in order of electrophoresis speed. After the release of CK-MB2 from the myocardium, the carboxyl terminal is hydrolyzed by plasma carboxyl peptidase N, and a lysine residue is removed to convert to the serum modified isoform CK-MB1. The increase of CK during myocardial injury is mainly caused by the increase of CKMB, which is manifested as the simultaneous increase of both. CK and CKMB are one of the sensitive indexes of myocardial tissue injury, especially CKMB is more specific.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (ng/mL)	50	25	12.5	6.25	3.12	1.56	0.78	0
OD	2.39	1.48	0.86	0.51	0.32	0.23	0.18	0.07
Corrected OD	2.32	1.41	0.79	0.44	0.25	0.16	0.11	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Mouse CKMB spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	84-101	96
Plasma(n=8)	92-105	102
Tissue Homogenates(n=8)	96-108	104

 

| Linearity

Samples were spiked with high concentrations of Mouse CKMB and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Serum(n=4)	Plasma(n=4)	Tissue Homogenates(n=4)
1 : 2	Range (%)	84-95	88-96	90-110
	Average (%)	91	93	96
1 : 4	Range (%)	89-103	87-107	105-115
	Average (%)	94	98	108