| FⅫ

Coagulation factor XII, also known as Hageman factor, is a plasma protein involved in coagulation. It is the zymogen form of factor XIIa , an enzyme of the serine protease (or serine endopeptidase) class. FXII is a plasma glycoprotein of approximately 9 kDa and is part of the coagulation cascade. It activates factor XI and prekallikrein in vitro. FXII is activated to FXIIa by negatively charged surfaces , initiating the intrinsic pathway. FXII also plays a role in diagnostic coagulation assays called activated partial thromboplastin times.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (pg/mL)	4000	2000	1000	500	250	125	62.5	0
OD	2.58	1.59	0.93	0.55	0.37	0.28	0.21	0.09
Corrected OD	2.49	1.5	0.84	0.46	0.28	0.19	0.12	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Mouse FⅫ spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Plasma(n=8)	92-105	101

 

| Linearity

Samples were spiked with high concentrations of Mouse FⅫ and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Plasma(n=4)
1 : 2	Range (%)	88-96
	Average (%)	93
1 : 4	Range (%)	86-108
	Average (%)	99