| FⅡ

Prothrombin (coagulation factor II) is encoded by the F2-gene. Structurally, it is a member of the large PA clan of proteases. It is proteolytically cleaved during the clotting process by the prothrombinase enzyme complex to form thrombin. In the blood coagulation pathway, thrombin acts to convert factor XI to XIa, VIII to VIIIa, V to Va, fibrinogen to fibrin, and XIII to XIIIa. In the conversion of fibrinogen into fibrin, thrombin catalyzes the cleavage of fibrinopeptides A and B from the respective Aα and Bβ chains of fibrinogen to form fibrin monomers.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (ng/mL)	200	100	50	25	12.5	6.25	3.12	0
OD	2.14	1.21	0.81	0.53	0.39	0.3	0.25	0.09
Corrected OD	2.05	1.12	0.72	0.44	0.3	0.21	0.16	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Mouse FⅡ spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Plasma(n=8)	90-105	100

 

| Linearity

Samples were spiked with high concentrations of Mouse FⅡ and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Plasma(n=4)
1 : 2	Range (%)	89-98
	Average (%)	94
1 : 4	Range (%)	87-108
	Average (%)	98