Product Name:
Mouse Fibrinogen Alpha Chain (FGA) ELISA Kit
Cat.#:
JL51648
Regulatory Status:
Alternative:
Fib2; FG-A; FGA; Fibrinopeptide A
Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
31.25-2000ng/mL
Sensitivity:
8.58ng/mL
Specificity:
This kit recognizes Mouse FGA in samples. No significant cross-reactivity or interference between Mouse FGA and analogues was observed.
Sample Type:
Plasma
Assay Procedure:

Research Area:
Cardiovascular Biology
Product Datasheets
| FGA
The fibrinogen alpha chain is a protein encoded by the FGA gene. The protein encoded by this gene is the alpha component of fibrinogen, a blood-borne glycoprotein composed of three pairs of nonidentical polypeptide chains. Mutations in this gene lead to several disorders, including dysfibrinogenemia, hypofibrinogenemia, afibrinogenemia, and renal amyloidosis. Alternative splicing results in two isoforms that vary in the carboxy-terminus.| Typical data
As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.Concentration (ng/mL) | 2000 | 1000 | 500 | 250 | 125 | 62.5 | 31.2 | 0 |
OD | 2.56 | 1.77 | 1.22 | 0.71 | 0.46 | 0.31 | 0.23 | 0.07 |
Corrected OD | 2.49 | 1.7 | 1.15 | 0.64 | 0.39 | 0.24 | 0.16 | - |
| Repeatability
Intra-assay Precision (Precision within an assay):CV% < 10%; Inter-assay Precision (Precision between assays):CV% < 10%| Recovery
The recovery of Mouse FGA spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.Sample Type | Range(%) | Average Recovery(%) |
Plasma(n=8) | 92-106 | 102 |
| Linearity
Samples were spiked with high concentrations of Mouse FGA and diluted with Universal Diluent to produce samples with values within the range of the assay.Plasma(n=4) | ||
1 : 2 | Range (%) | 88-98 |
Average (%) | 94 | |
1 : 4 | Range (%) | 88-108 |
Average (%) | 98 |






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