| GPR120

G-protein coupled receptor 12 (GPR12), also termed Free Fatty acid receptor 4 (FFAR4), is a protein encoded by the FFAR4 gene. G protein-coupled receptors (also termed GPRs or GPCRs) reside on their parent cells' surface membranes, bind any one of the specific set of ligands that they recognize, and thereby are activated to trigger certain responses in their parent cells. FFAR4 is able to regulate many normal body functions such as food preference, food consumption, food taste, body weight, blood sugar levels, inflammation, atherosclerosis and bone remodeling.Studies also suggest that the stimulation or suppression of FFAR4 alters the development and progression of several types of cancers.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (ng/mL)	20	10	5	2.5	1.25	0.62	0.31	0
OD	2.22	1.45	0.78	0.45	0.29	0.2	0.17	0.07
Corrected OD	2.15	1.38	0.71	0.38	0.22	0.13	0.1	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Mouse GPR120 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Tissue Homogenates(n=8)	84-102	96
Cell Lysates(n=8)	96-108	105

 

| Linearity

Samples were spiked with high concentrations of Mouse GPR120 and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Tissue Homogenates(n=4)	Cell Lysates(n=4)
1 : 2	Range (%)	84-95	90-110
	Average (%)	91	96
1 : 4	Range (%)	89-103	105-115
	Average (%)	94	108