| p-TAU181

The tau proteins form a group of six highly soluble protein isoforms produced by alternative splicing from the gene MAPT (microtubule-associated protein tau).Hyperphosphorylation of the tau protein can result in the self-assembly of tangles of paired helical filaments and straight filaments, which are involved in the pathogenesis of Alzheimer's disease, frontotemporal dementia and other tauopathies.P-tau181 is a variant of tau protein abnormally phosphorylated at the threonine 181 site.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (pg/mL)	100	50	25	12.5	6.25	3.12	1.56	0
OD	2.85	2.28	1.58	0.98	0.56	0.33	0.24	0.09
Corrected OD	2.76	2.19	1.49	0.89	0.47	0.24	0.15	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Monkey p-TAU181 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	84-101	97
Plasma(n=8)	92-106	101

 

| Linearity

Samples were spiked with high concentrations of Monkey p-TAU181 and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Serum(n=4)	Plasma(n=4)
1 : 2	Range (%)	84-94	88-97
	Average (%)	92	92
1 : 4	Range (%)	89-103	87-109
	Average (%)	93	96