| N6AMT1

N-6 adenine-specific DNA methyltransferase 1 is a protein encoded by the N6AMT1 gene. The N6AMT1 gene encodes an N(6)-adenine-specific DNA methyltransferase. The encoded enzyme may be involved in the methylation of release factor I during translation termination. This enzyme is also involved in converting the arsenic metabolite monomethylarsonous acid to the less toxic dimethylarsonic acid. Alternative splicing of this gene results in multiple transcript variants.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (ng/mL)	40	20	10	5	2.5	1.25	0.625	0
OD	2.18	1.26	0.77	0.45	0.33	0.24	0.19	0.08
Corrected OD	2.1	1.18	0.69	0.37	0.25	0.16	0.11	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Human N6AMT1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Tissue Homogenates(n=8)	83-101	96
Cell Lysates(n=8)	92-105	101

 

| Linearity

Samples were spiked with high concentrations of Human N6AMT1 and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Tissue Homogenates(n=4)	Cell Lysates(n=4)
1 : 2	Range (%)	83-95	88-97
	Average (%)	91	93
1 : 4	Range (%)	89-103	87-108
	Average (%)	94	100