| BMP-10

Bone morphogenetic protein 10 (BMP10) is a protein that in humans is encoded by the BMP10 gene.BMP10 is a polypeptide belonging to the TGF-β superfamily of proteins.It is a novel protein that, unlike most other BMP's, is likely to be involved in the trabeculation of the heart. Bone morphogenetic proteins are known for their ability to induce bone and cartilage development. BMP10 is categorized as a BMP since it shares a large sequence homology with other BMP's in the TGF-β superfamily.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (ng/mL)	10	5	2.5	1.25	0.62	0.31	0.15	0
OD	2.32	1.89	1.4	0.97	0.58	0.37	0.22	0.07
Corrected OD	2.25	1.82	1.33	0.9	0.51	0.3	0.15	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Human BMP-10 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	84-101	96
Plasma(n=8)	92-106	103
Tissue Homogenates(n=8)	96-108	105

 

| Linearity

Samples were spiked with high concentrations of Human BMP-10 and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Serum(n=4)	Plasma(n=4)	Tissue Homogenates(n=4)
1 : 2	Range (%)	84-95	88-97	90-110
	Average (%)	91	93	97
1 : 4	Range (%)	89-103	87-108	105-114
	Average (%)	94	98	107