Home > Sandwich ELISA Kits > Human DNA repair protein RAD51 homolog 1 (RAD51) ELISA Kit

Product Name:

Human DNA repair protein RAD51 homolog 1 (RAD51) ELISA Kit

Cat.#:

JL52464

Regulatory Status:

RUO

Alternative:

RAD51A; RECA; HsRad51; BRCC5; BRCA1/BRCA2-Containing Complex Subunit 5; RAD51 Homolog

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.312-20ng/mL

Sensitivity:

0.161ng/mL

Specificity:

This kit recognizes Human RAD51 in samples. No significant cross-reactivity or interference between Human RAD51 and analogues was observed.

Sample Type:

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Procedure:

Research Area:

Signal Transduction

Size & Price:

Human A1BG Polyclonal Antibody

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Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| RAD51

DNA repair protein RAD51 homolog 1 is a protein encoded by the gene RAD51. The enzyme encoded by this gene is a member of the RAD51 protein family which assists in repair of DNA double strand breaks. RAD51 family members are homologous to the bacterial RecA, Archaeal RadA, and yeast Rad51.The protein is highly conserved in most eukaryotes, from yeast to humans.The name RAD51 derives from RADiation sensitive protein 51.

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

Concentration (ng/mL)	20	10	5	2.5	1.25	0.625	0.312	0
OD	2.07	1.33	0.87	0.58	0.4	0.27	0.18	0.08
Corrected OD	1.99	1.25	0.79	0.5	0.32	0.19	0.1	-

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Human RAD51 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	83-101	96
Plasma(n=8)	92-105	102
Cell Culture Supernates(n=8)	97-108	104

| Linearity

Samples were spiked with high concentrations of Human RAD51 and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Serum(n=4)	Plasma(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	85-95	88-96	90-110
1 : 2	Average (%)	91	93	96
1 : 4	Range (%)	89-103	87-108	105-115
1 : 4	Average (%)	94	98	111