Home > Sandwich ELISA Kits > Human Retinol Binding Protein 3, Interstitial (RBP3) ELISA Kit

Product Name:

Human Retinol Binding Protein 3, Interstitial (RBP3) ELISA Kit

Cat.#:

JL52476

Regulatory Status:

RUO

Alternative:

RBP-3; IRBP; RBPI; Interphotoreceptor retinoid-binding protein; Interstitial retinol-binding protein

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.156-10ng/mL

Sensitivity:

0.072ng/mL

Specificity:

This kit recognizes Human RBP3 in samples. No significant cross-reactivity or interference between Human RBP3 and analogues was observed.

Sample Type:

Tissue homogenates, cell lysates and other biological fluids

Assay Procedure:

Research Area:

Signal Transduction

Size & Price:

Human A1BG Polyclonal Antibody

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Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| RBP3

Retinol-binding protein 3, interstitial (RBP3), also known as interphotoreceptor retinoid-binding protein (IRBP), is a protein that is encoded by the RBP3 gene.RBP3 orthologs have been identified in most eutherians except tenrecs and armadillos. A horizontal gene transfer from bacteria has been proposed to explain the evolution of the eye in chordates.

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

Concentration (ng/mL)	10	5	2.5	1.25	0.625	0.312	0.156	0
OD	2.28	1.45	0.84	0.5	0.33	0.24	0.18	0.08
Corrected OD	2.2	1.37	0.76	0.42	0.25	0.16	0.1	-

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Human RBP3 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Tissue Homogenates(n=8)	94-106	102
Cell Lysates(n=8)	96-108	103

| Linearity

Samples were spiked with high concentrations of Human RBP3 and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Tissue Homogenates(n=4)	Cell Lysates(n=4)
1 : 2	Range (%)	85-96	90-110
1 : 2	Average (%)	93	96
1 : 4	Range (%)	87-108	105-115
1 : 4	Average (%)	98	109