Human Toll Like Receptor 2 (TLR2) ELISA Kit

Cat.#:

JL14407

Regulatory Status:

RUO

Alternative:

CD282; TIL4; Toll/interleukin-1 receptor-like protein 4

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.31-20ng/mL

Sensitivity:

0.16ng/mL

Specificity:

This kit recognizes Human TLR2 in samples. No significant cross-reactivity or interference between Human TLR2 and analogues was observed.

Sample Type:

Saliva, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Procedure:

Research Area:

Cancer

Size & Price:

Human A1BG Polyclonal Antibody

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Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| TLR2

Toll-like receptor 2 also known as TLR2 is a protein that is encoded by the TLR2 gene.TLR2 has also been designated as CD282 (cluster of differentiation 282). TLR2 is one of the toll-like receptors and plays a role in the immune system. TLR2 is a membrane protein, a receptor, which is expressed on the surface of certain cells and recognizes foreign substances and passes on appropriate signals to the cells of the immune system.

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

Concentration (ng/mL)	20	10	5	2.5	1.25	0.62	0.31	0
OD	2.24	1.55	0.98	0.52	0.32	0.21	0.17	0.08
Corrected OD	2.16	1.47	0.9	0.44	0.24	0.13	0.09	-

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Human TLR2 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Tissue Homogenates(n=8)	84-101	96
Cell Lysates(n=8)	92-105	101
Cell Culture Supernates(n=8)	96-108	105

| Linearity

Samples were spiked with high concentrations of Human TLR2 and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Tissue Homogenates(n=4)	Cell Lysates(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	84-95	88-96	90-110
1 : 2	Average (%)	91	93	96
1 : 4	Range (%)	89-102	87-108	105-115
1 : 4	Average (%)	94	98	108