Human Matrix Metalloproteinase 7 (MMP-7) ELISA Kit

Cat.#:

JL11630

Regulatory Status:

RUO

Alternative:

MPSL1; MAT; PUMP-1; Matrilysin Uterine; Matrilysin; Matrin; Pump-1 Protease; Uterine Metalloproteinase

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.15-10ng/mL

Sensitivity:

0.05ng/mL

Specificity:

This kit recognizes Human MMP-7in samples. No significant cross-reactivity or interference between Human MMP-7 and analogues was observed.

Sample Type:

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Procedure:

Research Area:

Cancer

Size & Price:

Human A1BG Polyclonal Antibody

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Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| MMP-7

Matrilysin also known as matrix metalloproteinase-7 (MMP-7), pump-1 protease (PUMP-1), or uterine metalloproteinase is an enzyme in humans that is encoded by the MMP7 gene.The enzyme (EC 3.4.24.23) has also been known as matrin, putative (or punctuated) metalloproteinase-1, matrix metalloproteinase pump 1, PUMP-1 proteinase, PUMP, metalloproteinase pump-1, putative metalloproteinase, MMP).Human MMP-7 has a molecular weight around 30 kDa.

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

Concentration (ng/mL)	10	5	2.5	1.25	0.62	0.31	0.15	0
OD	1.99	1.26	0.82	0.52	0.37	0.27	0.22	0.08
Corrected OD	1.91	1.18	0.74	0.44	0.29	0.19	0.14	-

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Human MMP-7 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	84-101	96
Plasma(n=8)	92-105	102
Cell Culture Supernates(n=8)	96-108	105

| Linearity

Samples were spiked with high concentrations of Human MMP-7 and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Serum(n=4)	Plasma(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	84-95	88-96	90-110
1 : 2	Average (%)	91	93	96
1 : 4	Range (%)	89-103	87-108	104-115
1 : 4	Average (%)	94	98	108