Home > Sandwich ELISA Kits > Human 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 (PFKFB3) ELISA Kit

Product Name:

Human 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3 (PFKFB3) ELISA Kit

Cat.#:

JL46415

Regulatory Status:

RUO

Alternative:

6-phosphofructo-2-kinase;fructose-2,6-biphosphatase 3

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

15.6-1000pg/mL

Sensitivity:

7.9pg/mL

Specificity:

This kit recognizes Human PFKFB3 in samples. No significant cross-reactivity or interference between Human PFKFB3 and analogues was observed.

Sample Type:

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Procedure:

Research Area:

Metabolism

Size & Price:

Human A1BG Polyclonal Antibody

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Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| PFKFB3

PFKFB3 is a gene that encodes the 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 enzyme.It is one of 4 tissue-specific PFKFB isoenzymes identified currently (PFKFB1-4).

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

Concentration (pg/mL)	1000	500	250	125	62.5	31.2	15.6	0
OD	2.47	1.73	1.33	0.92	0.63	0.44	0.32	0.09
Corrected OD	2.38	1.64	1.24	0.83	0.54	0.35	0.23	-

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Human PFKFB3 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	84-101	96
Plasma(n=8)	92-105	102
Cell Culture Supernates(n=8)	96-108	105

| Linearity

Samples were spiked with high concentrations of Human PFKFB3 and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Serum(n=4)	Plasma(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	84-95	88-96	90-110
1 : 2	Average (%)	91	93	96
1 : 4	Range (%)	89-103	88-108	105-115
1 : 4	Average (%)	95	98	108