Home > Sandwich ELISA Kits > Human Receptor Activator Of Nuclear Factor Kappa B (RANκ) ELISA Kit

Product Name:

Human Receptor Activator Of Nuclear Factor Kappa B (RANκ) ELISA Kit

Cat.#:

JL19459

Regulatory Status:

RUO

Alternative:

RANκ

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.15-10ng/mL

Sensitivity:

0.05ng/mL

Specificity:

This kit recognizes Human RANκ in samples. No significant cross-reactivity or interference between Human RANκ and analogues was observed.

Sample Type:

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Procedure:

Research Area:

Signal Transduction

Size & Price:

Human A1BG Polyclonal Antibody

Email *

Message *

Please Sign In.

Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| RANκ

Nuclear factor κB receptor activator (RANκ), also known as TRANCE receptor or TNFRSF11A, is a member of the tumor necrosis factor receptor (TNFR) molecular subfamily. It is a receptor for RANK-ligand (RANKL) and is part of the RANK/RANKL/OPG signaling pathway that regulates osteoclast differentiation and activation. It is implicated in bone remodeling and repair, immune cell function, lymph node development, thermoregulation, and mammary gland development. Its cytoplasmic structural domain binds to TRAFs 1, 2, 3, 5, and 6 to signal to downstream targets such as NF-κB and JNK.

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

Concentration (ng/mL)	10	5	2.5	1.25	0.62	0.31	0.15	0
OD	2.48	1.45	0.93	0.54	0.36	0.24	0.16	0.06
Corrected OD	2.42	1.39	0.87	0.48	0.3	0.18	0.1	-

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Human RANκ spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Tissue Homogenates(n=8)	84-101	96
Cell Culture Supernates(n=8)	96-108	105

| Linearity

Samples were spiked with high concentrations of Human RANκ and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Tissue Homogenates(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	83-94	90-110
1 : 2	Average (%)	90	96
1 : 4	Range (%)	89-103	105-115
1 : 4	Average (%)	94	108