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Human Aggrecan (AGC) ELISA Kit

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Product Name:

Human Aggrecan (AGC) ELISA Kit

Cat.#:

JL18036

Regulatory Status:
Alternative:

ACAN; AGC1; AGCAN; CSPG1; CSPCP; CSPGCP; MSK16; SEDK; Cartilage-specific proteoglycan core protein; Large Aggregating Proteoglycan; Chondroitin Sulfate Proteoglycan 1

Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
0.46-30ng/mL
Sensitivity:
0.18ng/mL
Specificity:
This kit recognizes Human AGC in samples. No significant cross-reactivity or interference between Human AGC and analogues was observed.
Sample Type:
serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Assay Procedure:
Assay Procedure
Research Area:
Signal Transduction
  • Human Aggrecan (AGC) ELISA Kit
  • Human Aggrecan (AGC) ELISA Kit
  • Human Aggrecan (AGC) ELISA Kit
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Product Datasheets
Instruction: Down Instruction


| AGC

Aggrecan (ACAN), also known as cartilage-specific proteoglycan core protein (CSPCP) or chondroitin sulfate proteoglycan 1, is a protein that is encoded by the ACAN gene.This gene is a member of the lectican (chondroitin sulfate proteoglycan) family. The encoded protein is an integral part of the extracellular matrix in cartilagenous tissue and it withstands compression in cartilage.Aggrecan is a proteoglycan, or a protein modified with large carbohydrates; the human form of the protein is 2316 amino acids long and can be expressed in multiple isoforms due to alternative splicing.Aggrecan was named for its ability to form large aggregates in the cartilage tissue (a large aggregating proteoglycan).
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 
Concentration (ng/mL) 30 15 7.5 3.75 1.87 0.93 0.46 0
OD 2.48 1.31 0.93 0.52 0.33 0.22 0.16 0.07
Corrected OD 2.41 1.24 0.86 0.45 0.26 0.15 0.09 -
 





| Repeatability

Intra-assay Precision (Precision within an assay):CV% < 10%; Inter-assay Precision (Precision between assays):CV% < 10%
 

| Recovery

The recovery of Human AGC spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 
Sample Type Range(%) Average Recovery(%)
Serum(n=8) 84-101 97
Plasma(n=8) 92-105 102
Cell Culture Supernates(n=8) 98-108 105





 

| Linearity

Samples were spiked with high concentrations of Human AGC and diluted with Universal Diluent to produce samples with values within the range of the assay.

 
    Serum(n=4) Plasma(n=4) Cell Culture Supernates(n=4)
1 : 2 Range (%) 84-95 88-96 90-110
Average (%) 91 93 97
1 : 4 Range (%) 89-101 87-108 105-115
Average (%) 94 98 108







 
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Sample Data
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Sample Data
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Sample Data
Size Quantity Price ($)
48T
1
360 / 360
96T
1
460 / 460
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