| AGGF1

Angiogenic factor with G patch and FHA domains 1 is a protein that is encoded by the AGGF1 gene.AGGF1 functions as an angiogenic factor with a G-patch and forkhead-associated domain.This gene is predominantly expressed in activated, plump endothelial cells and acts to regulate angiogenesis and vascular development.AGGF1 is known to interact with a wide range of proteins involved in vascular development.Mutations to AGGF1 have been implicated in multiple cancers and is known to cause the rare congenital condition, Klippel-Trenaunay syndrome.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (ng/mL)	10	5	2.5	1.25	0.62	0.31	0.15	0
OD	2.51	1.58	0.94	0.61	0.38	0.26	0.23	0.08
Corrected OD	2.43	1.5	0.86	0.53	0.3	0.18	0.15	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Human AGGF1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	84-102	97
Plasma(n=8)	92-105	102
Tissue Homogenates(n=8)	96-108	105

 

| Linearity

Samples were spiked with high concentrations of Human AGGF1 and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Serum(n=4)	Plasma(n=4)	Tissue Homogenates(n=4)
1 : 2	Range (%)	84-95	88-95	90-110
	Average (%)	90	92	96
1 : 4	Range (%)	89-103	87-108	105-115
	Average (%)	94	98	108