Human Glucose Transporter 4 (GLUT4) ELISA Kit

Cat.#:

JL15045

Regulatory Status:

RUO

Alternative:

SLC2A4; Solute carrier family 2, facilitated glucose transporter member 4; Glucose transporter type 4, insulin-responsive

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.31-20ng/mL

Sensitivity:

0.11ng/mL

Specificity:

This kit recognizes Human GLUT4 in samples. No significant cross-reactivity or interference between Human GLUT4 and analogues was observed.

Sample Type:

Tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Procedure:

Research Area:

Signal Transduction

Size & Price:

Human A1BG Polyclonal Antibody

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Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| GLUT4

Glucose transporter type 4 (GLUT4), also known as solute carrier family 2, facilitated glucose transporter member 4, is a protein encoded by the SLC2A4 gene. GLUT4 is the insulin-regulated glucose transporter found primarily in adipose tissues and striated muscle (skeletal and cardiac). The first evidence for this distinct glucose transport protein was provided by David James in 1988.The gene that encodes GLUT4 was cloned and mapped in 1989.

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

Concentration (ng/mL)	20	10	5	2.5	1.25	0.62	0.31	0
OD	2.28	1.22	0.74	0.52	0.31	0.24	0.19	0.08
Corrected OD	2.2	1.14	0.66	0.44	0.23	0.16	0.11	-

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Human GLUT4 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Tissue Homogenates(n=8)	84-102	97
Cell Culture Supernates(n=8)	96-108	105

| Linearity

Samples were spiked with high concentrations of Human GLUT4 and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Tissue Homogenates(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	84-94	90-110
1 : 2	Average (%)	90	96
1 : 4	Range (%)	89-103	105-115
1 : 4	Average (%)	94	108