| ACSL3

Long-chain acyl-CoA synthase family member 3, also known as ACSL3, is an enzyme encoded by the ACSL3 gene. The protein encoded by this gene is an isoform within the long-chain fatty acid-CoA ligase family. Despite differences in substrate specificity, subcellular localization, and tissue distribution, all isoforms in this family convert free long-chain fatty acids into fatty acyl-CoA esters, thereby playing a crucial role in lipid biosynthesis and fatty acid degradation. This isoenzyme is highly expressed in the brain and preferentially utilizes myristic acid, arachidonic acid, and eicosapentaenoic acid as substrates. The amino acid sequence of this isoenzyme shares 92% identity with that of its rat homolog. Two transcript variants encoding the same protein have been identified for this gene.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (ng/mL)	5000	2500	1250	625	312	156	78	0
OD	2.36	1.78	1.23	0.79	0.46	0.29	0.17	0.09
Corrected OD	2.27	1.69	1.14	0.7	0.37	0.2	0.08	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Human ACSL3 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Tissue Homogenates(n=8)	84-101	96
Cell Lysates(n=8)	92-105	102

 

| Linearity

Samples were spiked with high concentrations of Human ACSL3 and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Tissue Homogenates(n=4)	Cell Lysates(n=4)
1 : 2	Range (%)	84-95	88-96
	Average (%)	88	93
1 : 4	Range (%)	89-100	87-108
	Average (%)	94	98