| CALP

Calprotectin is a complex of the mammalian proteins S100A8 and S100A9,with antimicrobial, proinflammatory and prothrombotic properties.Calprotectin is the only known antimicrobial manganese sequestration protein complex.Calprotectin comprises as much as 60% of the soluble protein content of the cytosol of a neutrophil, and it is secreted by an unknown mechanism during inflammation.Faecal calprotectin has been used to detect intestinal inflammation (colitis or enteritis) and can serve as a biomarker for inflammatory bowel diseases.Blood-based calprotectin (in serum and plasma) is used in diagnostics of multiple inflammatory diseases, including autoimmune diseases, like arthritis, and severe infections including sepsis.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (ng/mL)	720	360	180	90	45	22.5	11.25	0
OD	2.42	1.55	0.88	0.49	0.29	0.19	0.14	0.07
Corrected OD	2.35	1.48	0.81	0.42	0.22	0.12	0.07	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Human CALP spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Fecal(n=8)	84-101	96

 

| Linearity

Samples were spiked with high concentrations of Human CALP and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Fecal(n=4)
1 : 2	Range (%)	82-93
	Average (%)	91
1 : 4	Range (%)	89-103
	Average (%)	94