| B7-H3

B7 homolog 3 (B7-H3), also known as the CD276 molecule (CD276), is a protein encoded by the B7-H3 gene. B7-H3 is a 316 amino acid-long type I transmembrane protein that exists in two isoforms determined by its extracellular domain. The extracellular domain consists of a pair of immunoglobulin variable (IgV)-like and immunoglobulin constant (IgC)-like domains. Additionally, due to exon duplication, it comprises either one pair (2Ig-B7-H3) or two identical pairs (4Ig-B7-H3). B7-H3 mRNA is expressed in most normal tissues. In contrast, B7-H3 protein expression is very limited in normal tissues due to post-transcriptional regulation by microRNAs. However, B7-H3 protein is frequently overexpressed in many different cancer types.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

 

Concentration (pg/mL)	1500	750	375	187.5	93.75	46.87	23.44	0
OD	2.52	1.96	1.38	0.97	0.55	0.31	0.19	0.08
Corrected OD	2.44	1.88	1.3	0.89	0.47	0.23	0.11	-

 

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Human B7-H3 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	84-101	96
Plasma(n=8)	92-105	102
Cell Culture Supernates(n=8)	96-108	105

 

| Linearity

Samples were spiked with high concentrations of Human B7-H3 and diluted with Universal Diluent to produce samples with values within the range of the assay.

 

		Serum(n=4)	Plasma(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	84-95	88-96	90-110
	Average (%)	91	93	96
1 : 4	Range (%)	89-103	87-108	105-115
	Average (%)	94	98	108